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Faculty


Division of Basic Science

Lai-Xi Wang, Ph.D
Associate Professor
Institute of Human Virology and Department
of Biochemistry & Molecular Biology

lwang3@ihv.umaryland.edu

Profile Selected Publications
Education Research Interests
 

Photo: Lai-Xi Wang, Ph.D.

Profile

Dr. Lai-Xi Wang earned his Ph.D. in organic chemistry from Shanghai Institute of Organic Chemistry (SIOC), Chinese Academy of Sciences. During his graduate study, he spent 3 years at Japan's Institute of Physical & Chemical Research (RIKEN) learning the art of oligosaccharide synthesis. He did his postdoctoral studies with Prof. Y. C. Lee in glycobiology at Johns Hopkins University, then with Prof. Graham C. Walker in biochemistry and molecular biology at Massachusetts Institute of Technology (MIT).

Dr. Wang joined the faculty of the Institute of Human Virology as a tenure-track assistant professor in July 2000, and was promoted to associate professor with tenure in June 2005. Currently, he is an associate professor in the Department of Biochemistry & Molecular Biology, and a member of the Greenebaum Cancer Center in the University of Maryland School of Medicine.

Dr. Wang is also an executive editor for special issues for the journal Current Pharmaceutical Design (Bentham Science Publishers, USA). Dr. Wang was the recipient of the 2004 Young Investigator Award from the Division of Carbohydrate Chemistry, American Chemical Society. His research expertise is in bioorganic chemistry, synthetic carbohydrate chemistry, glycobiology, and immunology.
Education

•

Ph.D. - Organic Chemistry, SIOC, Chinese Academy of Science, 1991.
• Postdoctoral Fellow - Johns Hopkins University, Baltimore, MD, 1994-1997.
• Postdoctoral Associate - Massachusetts Institute of Technology, Cambridge, MA, 1997-2000.
Selected Publications
1. Wang, L. X., “Toward oligosaccharide- and glycopeptide-based HIV vaccines” (Invited review). Current Opinion in Drug Discovery and Development, 9, 194-206 (2006).
2. Ni. J., Song, H., Wang, Y., Stamatos, N., Wang, L. X. “Toward a carbohydrate-based HIV-1 vaccine: Synthesis and immunological studies of oligomannose-containing glycoconjugates”.
Bioconjugate Chemistry, 17, 493-500 (2006).
3. Zeng, Y., Wang, J., Li, B., Hauser, S., Li, H., Wang, L. X. “Glycopeptide synthesis through endoglycosidase-catalyzed oligosaccharide transfer of sugar oxazolines. Probing substrate structural requirement. Chemistry: A European Journal, 12, 3355-3364 (2006).
4. Li, H, Li, B., Song, H., Breydo, L., Baskakov, I. V., Wang, L. X. “Chemoenzymatic synthesis of HIV-1 V3 domain glycopeptides carrying two N-Glycans and effects of glycosylation on the peptide’s domain”. Journal of Organic Chemistry, 70, 9990-9996 (2005)
5. Li, B., Zeng, Y., Hauser, S., Song, H., Wang, L. X. “Highly efficient endoglycosidase-catalyzed synthesis of glycopeptides using oligosaccharide oxazolines as donor substrates”. Journal of the American Chemical Society, 127, 9692-9693 (2005).
6. Wang, J., Le, N., Heredia, A., Song, H., Redfield, R., Wang, L. X. “Modification and structure-activity relationship of a small molecule HIV-1 inhibitor targeting the viral envelope glycoprotein gp120”. Organic & Biomolecular Chemistry, 3, 1781-1786 (2005).
7. Stamatos, N. M., Liang, F., Nan, X., Landry, K., Cross, A. S., Wang, L. X. & Pshezhetsky, A. V. “Endogeneous sialidase activity of human monocytes is up-regulated during cellular differentiation into macrophages”. FEBS Journal, 272, 2545-2556 (2005).
8. Wang, L. X., Song, H., Liu, S., Lu, H., Jiang, S., Ni, J., Li, H. “Chemoenzymatic synthesis of HIV-1 glycopeptides. Effects of glycosylation on the antiviral activity and a-helix bundle-forming ability of gp41 peptide C34”. ChemBioChem, 6, 1068-1074 (2005).
9. Hauser, S., Song, H., Li, H., Wang, L. X. “A novel fluorescence-based assay for the transglycosylation activity of endo-beta-N-acetyl-glucosaminidases”. Biochemical & Biophysical Research Communications, 328, 580-585 (2005).
10. Li, H., Singh, S., Song, H., Zeng, Y., Wang, L. X. “Chemoenzymatic synthesis of CD52 glycoprotein carrying native N-glycans”. Bioorganic and Medicinal Chemistry Letters, 15, 895-898 (2005).
11. Wang, L. X., Heredia, A., Song, H., Zhang, Z., Yu, B., Davis, C. & Redfield, R. “Resveratrol glucuronides as the metabolites of resveratrol in humans: characterization, synthesis, and anti-HIV activities”. Journal of Pharmaceutical Sciences, 93, 2448-2457 (2004).
12. Li, H., Song, H., Heredia, A., Le, N., Redfield, R., Lewis, G. K., & Wang, L. X. “Synthetic bivalent CD4-mimetic miniproteins show enhanced anti-HIV activity over the monovalent miniprotein”. Bioconjugate Chemistry, 15, 783-789 (2004).
13. Ni, J., Powell, R., Baskakov, I. V., DeVico, A., Lewis, G. K. & Wang, L. X. “Synthesis, conformation, and immunogenicity of monosaccharide-centered multivalent HIV-1 gp41 peptides containing the sequence of DP178”. Bioorganic & Medicinal Chemistry, 12, 3141-3148 (2004).
14. Naicker, K. P., Li, H., Heredia, A., Song, H., & Wang, L. X. Design and synthesis of alpha-Gal-conjugated peptide T20 as novel antiviral agent for HIV-immunotargeting. Organic & Biomolecular Chemistry, 2, 660-664 (2004). (This paper was featured in Chemical Science as “one of the hottest papers”, Chem. Sci., 2004, 1, C26)
15. Wang, L. X., Ni, J., Singh, S. & Li, H. “Binding of high-mannose type oligosaccharides and synthetic oligomannose clusters to human antibody 2G12. Implications for HIV-1 vaccine design”. Chemistry & Biology, 11, 127-134 (2004).
16. Li, H. & Wang, L. X. “Design and synthesis of a cholic acid-based oligomannose cluster as the epitope mimic for human HIV-neutralizing antibody 2G12”. Organic & Biomolecular Chemistry, 2, 483-488 (2004).
(This paper was featured in Chemical Science as “one of the hottest papers”, Chem. Sci., 2004, 1, C20)
17. Naicker, K. P., Jiang, S., Lu, H., Ni, J., Boyer-Chatenet, L., Wang, L. X. & Debnath, A. K. “Synthesis and anti-HIV-1 activity of 4-[4-(4,6-bisphenylamino-[1,3,5]triazin-2-ylamino)-5-methoxy-2-methylphenylazo]-5-hydroxynaphthalene-2,7-disulfonic acid and its derivatives”. Bioorganic & Medicinal Chemistry, 12, 1215-1220 (2004).
18. Locke, D, Bevans, C., Wang, L. X., Zhang, Y., Harris, A. & Lee, Y. C. “Neutral, acidic and basic amide derivatives of anthranilamide for labeling of reducing oligosaccharides”. Carbohydrate Research, 339, 221-231 (2004).
19. Snaar-Jagalska B. E., Krens, S. F., Robina, I., Wang, L. X. & Spaink, H. P. “Specific activation of ERK pathways by chitin oligosaccharides in embryonic zebrafish cell lines”. Glycobiology, 13, 725-32 (2003).
20. Li, H. & Wang, L. X. “Cholic acid as template for multivalent peptide assembly”. Organic & Biomolecular Chemistry, 1, 3507-13 (2003).
21. Wang, L. X. “Bioorganic approaches towards HIV vaccine design” (Invited review). Current Pharmaceutical Design, 9, 1771-1787 (2003).
22. Cross, A. S., Sakarya, S., Rifat, S., Held, T. K., Drysdale, B. E., Grange, P. A., Cassels, F. J., Wang, L. X., Stamatos, N. M., Farese, A., Casey, D., Powell, J., Bhattacharjee, A. K., Kleinberg, M. & Goldblum, S. E. “Recruitment of murine neutrophils in vivo through endogenous sialidase activity”. Journal of Biological Chemistry, 278, 4112-20 (2003).
23. Ni, J., Singh, S. & Wang, L. X. “Synthesis of maleimide-activated carbohydrates as chemoselective tags for site-specific glycosylation of peptides and proteins”. Bioconjugate Chemistry, 14, 232-38 (2003).
24. Singh, S., Ni, J. & Wang, L. X. “Chemoenzymatic synthesis of high-mannose type HIV-1 gp120 glycopeptides”. Bioorganic & Medicinal Chemistry Letters, 13, 327-30 (2003).
25. Wang, L. X., Ni, J. & Singh, S. “Carbohydrate-centered maleimide cluster as new types of templates for multivalent peptide assembling: Synthesis of multivalent HIV-1 gp41 peptides”. Bioorganic & Medicinal Chemistry, 11, 129-36 (2003).
26. Park, J. K., Wang, L. X., Patel, H. V. & Roseman, S. “Molecular cloning and characterization of a unique beta -glucosidase from vibrio cholerae”. Journal of Biological Chemistry, 277, 29555-60 (2002).
27. Park, J. K., Wang, L. X. & Roseman, S. “Isolation of a glucosamine-specific kinase, a unique enzyme of Vibrio cholerae”. Journal of Biological Chemistry, 277, 15573-78. (2002).
28. Southwick, A. M., Wang, L. X., Long, S. R. & Lee, Y. C. “Activity of Sinorhizobium meliloti NodAB and NodH enzymes on thiochitooligosaccharides”. Journal of Bacteriology, 184, 4039-43. (2002).

Research Interests
Click here to view Dr. Wang's research interests.
University of Maryland Biotechnology InstituteUniversity of Maryland Biotechnology InstituteUniversity of Maryland Medical System The Institute of Human Virology
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