Faculty
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Division of Basic Science
Wuyuan Lu, Ph.D.
Associate Professor
Laboratory of Synthetic Protein Chemistry
wlu@ihv.umaryland.edu
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| Profile |
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Dr. Lu obtained his Ph.D. in protein biophysical chemistry from Purdue University and trained as a postdoctoral fellow in synthetic peptide chemistry at the Scripps Research Institute.
After a brief tenure as a senior staff scientist and group leader in protein biochemistry at Gryphon Sciences, Inc., and a research associate (assistant professor) at the University of Chicago, Dr. Lu joined the Institute of Human Virology in July 2000 as a tenure-track assistant professor of the Basic Science Division, and was promoted to associate professor with tenure in June 2005.
Dr. Lu is also an affiliate associate professor and a member on the graduate faculty in the Department of Chemistry and Biochemistry, University of Maryland Baltimore County.
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| Education |
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B.S. - Chemistry, Nanjing University, China, 1984
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Ph.D. - Chemistry, Purdue University, West Lafayette, 1994. |
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Postdoctoral Fellow - The Scripps Research Institute, La Jolla. |
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Research Associate (Assistant Professor) - The University of Chicago, Chicago |
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| Selected Publications |
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| 1. |
Wu, Z., Powell, R. and Lu, W. (2003). Productive folding of human neutrophil a-defensins in vitro without the pro-peptide. J. Am. Chem. Soc. 125, 2402-3. |
| 2. |
Pál, G., Santamaria, F., Kossiakoff, A. and Lu, W. (2003). The first semi-synthetic serine protease made by native chemical ligation. Protein Expres. Purif. 29, 185-92. |
| 3. |
Santamaria, F., Wu, Z., Boulegue, C., Pal, G. and Lu, W. (2003). Reexamination of the recognition preference of the specificity pocket of the Abl SH3 domain by targeted synthetic peptide libraries. J. Mol. Recognit. 16, 131-8. |
| 4. |
Wu, Z., Prahl, A., Powell, R., Ericksen, B., Lubkowski, J. and Lu, W. (2003). From pro defensins to defensins: synthesis and characterization of human neutrophil pro a-defensin-1 and its mature domain. J. Pept. Res. 62, 53-62. |
| 5. |
Qasim, M.A., Lu, W., Lu, S., Ranjbar, M., Yi, Z-P, Chiang, Y-W, Ryan, K., Anderson, S., Zhang, W., Qasim, S. and Laskowski, M. Jr. (2003). Testing of the additivity based protein sequence to reactivity algorithm. Biochemistry 42, 6460-6. |
| 6. |
Asojo, O.A., Boulegue, C., Hoover, D.M., Lu, W. and Lubkowski, J. (2003). High-resolution crystal structures of thymus and activation-regulated chemokine (TARC). Acta Crystallogr. D59, 1165-1173. |
| 7. |
Wu, Z., Hoover, D.M., Yang, D., Boulegue, C., Santamaria, F., Oppenheim, J., Lubkowski, J. and Lu, W. (2003). Engineering disulfide bridges to dissect antimicrobial and chemotactic activities of human b-defensin 3. Proc. Natl. Acad. Sci. USA 100, 8880-8885. |
| 8. |
Hoover, D.M., Wu, Z., Tucker, K., Lu, W. and Lubkowski, J. (2003). Antimicrobial characterization of human b-defensin 3. Antimicrob. Agents Chemother. 47, 2804-2809. |
| 9. |
Cai, Z., Xu, C., Xu, Y., Lu, W., Chi, C-W., Shi, Y. and Wu, J. (2004). Solution structure of BmBKTx1, a new BKCa1 channel blocker from the Chinese scorpion Buthus martensi Karsch. Biochemsitry 43, 3764-3771. |
| 10. |
Szyka, A., Lu, W., Xu, C. and Lubkowski, J. (2004). Structure of the scorpion toxin BmBKTx1 solved from single wavelength anomalous scattering of sulfur. J. Struct. Biol. 145, 289-294. |
| 11. |
Xu, C.Q., Brone, B., Wicher, D., Bozkurt, O., Lu, W., Huys, I., Han, Y.H., Tytgat, J., Van Kerkhove, E., Chi, C.W. (2004). BmBKTx1, a novel Ca2+-activated K+ channel blocker purified from the Asian scorpion Buthus martensi Karsch. J. Biol. Chem. 279, 34562-34569. |
| 12. |
Wu, Z., Ericksen, B., Tucker, K., Lubkowski, J. and Lu, W. (2004). Synthesis and characterization of human a-defensins 4-6. J. Pept. Res. 64, 118-125. |
| 13. |
Wu, Z., Alexandratos, J., Ericksen, B., Lubkowski, J., Gallo, R.C. and Lu, W. (2004). Total chemical synthesis of N-myristoylated HIV-1 matrix protein p17: Structural and mechanistic implications of p17 myristoylation. Proc. Natl. Acad. Sci. USA 101, 11587-11592. |
| 14. |
Ericksen, B., Wu, Z., Lu, W. and Lehrer, R.I. (2005). Bactericidal activities and specificities of all six synthetic human a-defensins. Antimicrob. Agents Chemother. 49, 269-275. |
| 15. |
Wu, Z., Cocchi, F., Gentles, D., Ericksen, B., Lubkowski, J., DeVico, A., Lehrer, R.I. and Lu, W. (2005). Human neutrophil a-defensin 4 inhibits HIV-1 infection in vitro. FEBS Lett. 579, 162-166. |
| 16. |
Wang, C.G., Cai, Z., Lu, W., Wu, J., Xu, Y., Shi, Y., and Chi, C.W. (2005). A novel short-chain peptide BmKX from the Chinese scorpion Buthus martensi Karsch, sequencing, gene cloning and structure determination. Toxicon 45, 309-19. |
| 17. |
Xie, C., Prahl, A., Ericksen, B., Wu, Z., Zeng, P., Li, Q., Lu, W-Y., Lubkowski, J., and Lu, W. (2005). Reconstruction of the conserved b-bulge in mammalian defensins using D-amino acids. J. Biol. Chem. 280, 32921-32929. |
| 18. |
Lim, J.K., Burns, J.M., Lu, W. and DeVico, A.L. (2005). Characterization of post-translational RANTES/CCL5 processing by human peripheral blood mononuclear cells: elucidation of a novel 4-68 isoform. J. Leukoc. Biol. 78, 442-52. |
| 19. |
Xie, C., Zeng, P., Ericksen, B., Wu, Z., Lu, W-Y., and Lu, W. (2005). Effects of the terminal charges in human neutrophil a-defensin 2 on its bactericidal and membrane activity. Peptides 26, 2377-83. |
| 20. |
John, M., Keller, M.J., Fam, E.H., Cheshenko, N., Hogarty, K., Kasowitz, A., Wallenstein, S., Carlucci, M.J., Tuyama, A.C., Lu, W., Lehrer, R.I., Klotman, M.E., and Herold, B.C. (2005). Cervicovaginal secretions contribute to innate resistance to HSV infection. J. Infect. Dis. 192, 1731-40. |
| 21. |
Economopoulou, M., Bdeir, K., Cines, D.B., Fogt, F., Bdeir, Y., Lubkowski, J., Lu, W., Preissner, K.T., Hammes, H-P., and Chavakis, T. (2005). Inhibition of pathological retinal neovascularization by a-defensins. Blood 106, 3831-8. |
| 22. |
Sun, L., Finnegan, C., Kish-Catalone, T., Blumenthal, R., Garzino-Demo, P., La Terra Maggiore, G.M., Berrone, S., Kleinman, C., Wu, Z., Abdelwahab, S., Lu, W., and Garzino-Demo, A. (2005). Human b-defensins suppress HIV infection: potential role in mucosal protection. J. Virol. 79, 14318-29. |
| 23. |
Li, X., de Leeuw, E., and Lu, W. (2005). Total chemical synthesis of human psoriasin by native chemical ligation. Biochemistry 44, 14688-94. |
| 24. |
Wu, Z., Li, X., de Leeuw, E., Ericksen, B., and Lu, W. (2005). Why is the Arg5-Glu13 salt bridge conserved in mammalian a-defensins? J. Biol. Chem. 280, 43039-43047. |
| 25. |
Wang, C.Z., Zhang, H., Jiang, H., Lu, W., Zhao, Z.Q., and Chi, C.W. (2006). A novel conotoxin from Conus striatus, mu-SIIIA, selectively blocking rat tetrodotoxin-resistant sodium channels. Toxicon 47, 122-32.
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| 26. |
Yi, ZP., Vitek, O., Qasim, M.A., Lu, S.M., Lu, W., Ranjbar, M., Li, J., Laskowski, M.C., Bailey-Kellogg, C., and Laskowski, M., Jr. (2006). Functional evolution within a protein superfamily. Proteins 63, 697-708. |
| 27. |
Buck, C.B., Day, P.M., Thompson, C.D., Lubkowski, J., Lu, W., Lowy, D.R., and Schiller J.T. (2006). Human alpha-defensins block papillomavirus infection. Proc. Natl. Acad. Sci. USA 103, 1516-21. |
| 28. |
Kish-Catalone, T.M., Lu, W., Gallo, R.C., and DeVico, A.L. (2006). Preclinical evaluation of synthetic –2 RANTES as a candidate vaginal microbicide to target CCR5. Antimicrob. Agents Chemother. 50, 1497-509. |
| 29. |
Pazgier, M., Hoover, D.M., Yang, D., Lu, W., and Lubkowski, J. (2006). Human b-defensins. Cell. Mol. Life Sci. (in press) |
| 30. |
De Leeuw, E., Li, X., and Lu, W. (2006). Binding characteristics of the Lactobacillus brevis ATCC 8287 surface layer to extra cellular matrix proteins. FEMS Microbiol. Lett. (in press) |
| 31. |
Aono, S., Li, C., Zhang, G., Kemppainen, R.J., Gard, J., Lu, W., Hu, X., Schwartz, D.D., Morrison, E.E., Dykstra, C., and Shi, J. (2006). Molecular and functional characterization of bovine b-defensin-1. Vet. Immunol. Immunopathol. (in press) |
| 32. |
Wang, W., Mulakala, C., Ward, S.C., Jung, G., Luong, H., Pham, D., Waring, A.J., Kaznessis, Y., Lu, W., Bradley, K.A., and Lehrer, R.I. (2006). Retrocyclins kill the bacilli and spores of Bacillus anthracis and inactivate its lethal toxin. J. Biol. Chem. (in press) |
| 33. |
Szyk, A., Wu, Z., Tucker, K., Yang, D., Lu, W., and Lubkowski, J. (2006). Crystal structures of human a-defensins HNP4, HD5 and HD6. Protein Sci. (in press)
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| Research Interests |
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| Click here to view Dr. Lu's Research Interests |
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